BK 초청세미나 (건국대, 이충환교수)
생명과학과
2012-01-19
세미나 장소 : J215
세미나 일시 : 2012.01.19. (16:30)
세미나 발표자 : 건국대, 이충환교수.
세미나 일시 : 2012.01.19. (16:30)
세미나 발표자 : 건국대, 이충환교수.
Metabolomic Applications for the Screening of Novel Bioactive Compounds and Identification of Metabolic Changes
The metabolomics is the studies on metabolites, and by-products of the chemical reactions that continuously go on in every biological system. Metabolomics, the chemical profiling of (all) cellular metabolites by their identification and quantification, is a rapidly expanding strategy in the post-genomics era complementing transcriptomics and proteomics thereby constituting a trilogy.
A searchable library of MS/MS spectra, obtained using a quadrupole ion trap mass spectrometer and electrospray ionization, is presented for metabolomic profiling of secondary metabolite. The application of wideband excitation and normalized collision energy leads to highly reproducible mass spectra which are searched using the NIST algorithm. The ability to obtain library searchable spectra is demonstrated for the analysis of 5,000 secondary metabolites spectrum data.
In the fermentation of Phomopsis longicolla S1B4, metabolite patterns could be clearly differentiated based on fermentation time (phase 1 (days 4-8) and phase 2 (days 10-20)), 6 secondary and 10 primary metabolites were significantly contributed the clustering. The five secondary metabolites including identified novel compound, monodeacetylphomoxanthone B, were strongly positively correlated with antibacterial activity against Xanthomonas oryzae (Xoo) during fermentation.
The microbial community and metabolites differences in faecal samples of IBS (Irritable Bowel Syndrome) and non-IBS Korean subjects demonstrated a clear discrepancy between the faecal microbial diversity and richness measured as the presence of 16S rRNA gene signatures of bacteria and their metabolic activities. Key bacterial communities were shown to be significantly different in diversity and dominance between IBS and nIBS samples. The diversity and quantity were interlinked only in the Bacteroidetes, Bifidobacteria and Lactobacillus groups. The higher levels of amino acids and phenolic compounds indicated increased bacterial metabolism and also altered energy by the predominant gut microbiota.
This metabolomics study provides valuable information in regards to optimizing the cultivation process for bioactive compound production and describes an efficient way to search for novel bioactive compounds. The combined mass spectrometry approaches used in this study may be useful in understanding the overall metabolism in the various microbes.
A searchable library of MS/MS spectra, obtained using a quadrupole ion trap mass spectrometer and electrospray ionization, is presented for metabolomic profiling of secondary metabolite. The application of wideband excitation and normalized collision energy leads to highly reproducible mass spectra which are searched using the NIST algorithm. The ability to obtain library searchable spectra is demonstrated for the analysis of 5,000 secondary metabolites spectrum data.
In the fermentation of Phomopsis longicolla S1B4, metabolite patterns could be clearly differentiated based on fermentation time (phase 1 (days 4-8) and phase 2 (days 10-20)), 6 secondary and 10 primary metabolites were significantly contributed the clustering. The five secondary metabolites including identified novel compound, monodeacetylphomoxanthone B, were strongly positively correlated with antibacterial activity against Xanthomonas oryzae (Xoo) during fermentation.
The microbial community and metabolites differences in faecal samples of IBS (Irritable Bowel Syndrome) and non-IBS Korean subjects demonstrated a clear discrepancy between the faecal microbial diversity and richness measured as the presence of 16S rRNA gene signatures of bacteria and their metabolic activities. Key bacterial communities were shown to be significantly different in diversity and dominance between IBS and nIBS samples. The diversity and quantity were interlinked only in the Bacteroidetes, Bifidobacteria and Lactobacillus groups. The higher levels of amino acids and phenolic compounds indicated increased bacterial metabolism and also altered energy by the predominant gut microbiota.
This metabolomics study provides valuable information in regards to optimizing the cultivation process for bioactive compound production and describes an efficient way to search for novel bioactive compounds. The combined mass spectrometry approaches used in this study may be useful in understanding the overall metabolism in the various microbes.